› 1lqu

X-ray diffraction
1.25Å resolution

Mycobacterium tuberculosis FprA in complex with NADPH

Released:
DOI: 10.2210/pdb1lqu/pdb
PDB entry 1lqu coloured by chain, front view.
PDB entry 1lqu coloured by chain, side view.
PDB entry 1lqu coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
A covalent modification of NADP+ revealed by the atomic resolution structure of FprA, a Mycobacterium tuberculosis oxidoreductase.
Bossi RT, Aliverti A, Raimondi D, Fischer F, Zanetti G, Ferrari D, Tahallah N, Maier CS, Heck AJ, Rizzi M, Mattevi A
Biochemistry 41 8807-18 (2002)
PMID: 12102623

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-ferredoxin reductase FprA Chains: A, B
Molecule details ›
Chains: A, B
Length: 456 amino acids
Theoretical weight: 49.4 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIQ3 (Residues: 1-456; Coverage: 100%)
Gene names: MTCY164.16, Rv3106, fprA
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NDP 2 x NDP
1 bound ligand:
Ligand ACT 11 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 69.507Å b: 89.466Å c: 161.658Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.126 0.142
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structural and functional diversity of ferredoxin-NADP(+) reductases.
Aliverti et al. (2008)
10 more

1 mention without citation

Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes.
Plapp et al. (2012)