1lpq

X-ray diffraction
3.14Å resolution

Human DNA Topoisomerase I (70 Kda) In Non-Covalent Complex With A 22 Base Pair DNA Duplex Containing an 8-oxoG Lesion

Released:
Source organism: Homo sapiens
Primary publication:
8-Oxoguanine rearranges the active site of human topoisomerase I.
Proc. Natl. Acad. Sci. U.S.A. 99 12102-7 (2002)
PMID: 12209008

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 564 amino acids
Theoretical weight: 66.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11387 (Residues: 202-765; Coverage: 74%)
Gene name: TOP1
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*(8OG)P*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*CP*AP*AP*GP*TP*CP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 57.2Å b: 122.5Å c: 72Å
α: 90° β: 97.4° γ: 90°
R-values:
R R work R free
0.256 0.256 0.298
Expression systems:
  • Spodoptera frugiperda
  • Not provided