X-ray diffraction
2.38Å resolution

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.84 KDa
Source organism: Simian rotavirus A/SA11
Expression system: Escherichia coli
  • Canonical: P03536 (Residues: 206-315; Coverage: 35%)
Structure domains: Nonstructural RNA-binding protein
Eukaryotic translation initiation factor 4 gamma 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 amino acids
Theoretical weight: 3.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q04637 (Residues: 172-199; Coverage: 2%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 59.69Å b: 74.04Å c: 77.46Å
α: 90° β: 90° γ: 90°
R R work R free
0.221 0.221 0.272
Expression systems:
  • Escherichia coli
  • Not provided