1lgq

X-ray diffraction
2.1Å resolution

Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CHFR Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 12.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96EP1 (Residues: 14-124; Coverage: 17%)
Gene names: CHFR, RNF196
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 54.9Å b: 52.9Å c: 77.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.242 0.288
Expression system: Escherichia coli BL21