1ldd

X-ray diffraction
2Å resolution

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF Ubiquitin Ligase Complex

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaphase-promoting complex subunit 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 74 amino acids
Theoretical weight: 8.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12440 (Residues: 773-846; Coverage: 9%)
Gene names: APC2, L3105, L3108, RSI1, YLR127C
Sequence domains: Anaphase promoting complex (APC) subunit 2
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 54.7Å b: 72.9Å c: 79.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.266
Expression system: Escherichia coli