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LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY

X-ray diffraction
4.8Å resolution
PDB entry 1lbg coloured by chain, front view.
PDB entry 1lbg coloured by chain, side view.
PDB entry 1lbg coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Lactose operon repressor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 7901.92 Å2
Buried surface area: 1881.74 Å2
Dissociation area: 940.87 Å2
Dissociation energy (ΔGdiss): 16.87 kcal/mol
Dissociation entropy (TΔSdiss): 10.89 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114128
Assembly 2
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Assembly Name: Lactose operon repressor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 7592.92 Å2
Buried surface area: 2281.31 Å2
Dissociation area: 1,140.66 Å2
Dissociation energy (ΔGdiss): 16.57 kcal/mol
Dissociation entropy (TΔSdiss): 10.9 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114128

Macromolecules

Lactose operon repressor
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 38.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-360; Coverage: 100%)
Gene names: JW0336, b0345, lacI
Pfam:
InterPro:
SCOP:
Lactose operon repressor in PDB entry 1lbg, assembly 1, front view.
Lactose operon repressor in PDB entry 1lbg, assembly 1, side view.
Lactose operon repressor in PDB entry 1lbg, assembly 1, top view.
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DNA chains E, F, G, H
Name: DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3')
Representative chains: E, F, G, H
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') in PDB entry 1lbg, assembly 1, front view.
DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') in PDB entry 1lbg, assembly 1, side view.
DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') in PDB entry 1lbg, assembly 1, top view.
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