1l9e

X-ray diffraction
1.85Å resolution

Role of Histidine 269 in Catalysis by Monomeric Sarcosine Oxidase

Released:
Source organism: Bacillus sp. B-0618
Primary publication:
Monomeric sarcosine oxidase: role of histidine 269 in catalysis.
Biochemistry 41 9751-64 (2002)
PMID: 12146941

Function and Biology Details

Reaction catalysed:
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 73.083Å b: 70.638Å c: 74.297Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.168 0.168 0.195
Expression system: Escherichia coli