Solution NMR

Solution NMR Structure of BRCT Domain of T. Thermophilus: Northeast Structural Genomics Consortium Target WR64TT

Source organism: Thermus thermophilus
Entry authors: Sahota G, Dixon BL, Huang YP, Aramini J, Monleon D, Bhattacharya D, Swapna GVT, Yin C, Xiao R, Anderson S, Tejero R, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed:
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.03 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P26996 (Residues: 588-676; Coverage: 13%)
Gene names: TTHA1097, lig, ligA, ligT
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing
Chemical shifts: BMR5328  
Expression system: Escherichia coli BL21(DE3)