1l5h

X-ray diffraction
2.3Å resolution

FeMo-cofactor Deficient Nitrogenase MoFe Protein

Released:

Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 55.23 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07328 (Residues: 2-492; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains: Nitrogenase molybdenum iron protein domain
Nitrogenase molybdenum-iron protein beta chain Chain: B
Molecule details ›
Chain: B
Length: 522 amino acids
Theoretical weight: 59.4 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07329 (Residues: 2-523; Coverage: 100%)
Gene name: nifK
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 150.452Å b: 191.922Å c: 102.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.289