› 1l4a

X-ray diffraction
2.95Å resolution

X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI

Released:
DOI: 10.2210/pdb1l4a/pdb
PDB entry 1l4a coloured by chain, front view.
PDB entry 1l4a coloured by chain, side view.
PDB entry 1l4a coloured by chain, top view.
Source organism: Doryteuthis pealeii
Primary publication:
X-ray structure of a neuronal complexin-SNARE complex from squid.
Bracher A, Kadlec J, Betz H, Weissenhorn W
J Biol Chem 277 26517-23 (2002)
PMID: 12004067

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-129213 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Synaptobrevin Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 9.05 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: P47194 (Residues: 25-104; Coverage: 64%)
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
t-SNARE coiled-coil homology domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 9.98 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: O46345 (Residues: 183-265; Coverage: 28%)
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein Chain: C
Molecule details ›
Chain: C
Length: 83 amino acids
Theoretical weight: 9.57 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8T3S4 (Residues: 4-86; Coverage: 39%)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein Chain: D
Molecule details ›
Chain: D
Length: 87 amino acids
Theoretical weight: 9.67 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8T3S4 (Residues: 126-212; Coverage: 41%)
Sequence domains: SNAP-25 family
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Complexin Chain: E
Molecule details ›
Chain: E
Length: 79 amino acids
Theoretical weight: 9.25 KDa
Source organism: Doryteuthis pealeii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95PA1 (Residues: 25-98; Coverage: 49%)
Gene name: cpx
Sequence domains: Synaphin protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 26.072Å b: 75.43Å c: 243.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.3 0.297 0.344
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Molecular machines governing exocytosis of synaptic vesicles.
Jahn et al. (2012)
23 more

7 mentions without citation

The molecular signature of spermatogonial stem/progenitor cells in the 6-day-old mouse testis.
Kokkinaki et al. (2009)
6 more