PDBe 1l0a

X-ray diffraction
2.9Å resolution

DOWNSTREAM REGULATOR TANK BINDS TO THE CD40 RECOGNITION SITE ON TRAF3

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TNF receptor-associated factor 3 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13114 (Residues: 377-568; Coverage: 34%)
Gene names: CAP1, CRAF1, TRAF3
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
TRAF family member-associated NF-kappa-B activator Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 2.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92844 (Residues: 178-194; Coverage: 4%)
Gene names: ITRAF, TANK, TRAF2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P321
Unit cell:
a: 83Å b: 83Å c: 76.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.295
Expression system: Escherichia coli