X-ray diffraction
3.1Å resolution

Mutant Enzyme L119F Lumazine Synthase from S.pombe


Function and Biology Details

Reaction catalysed:
1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H(2)O + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo pentamer (preferred)
homo decamer
Entry contents:
1 distinct polypeptide molecule
6,7-dimethyl-8-ribityllumazine synthase Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 159 amino acids
Theoretical weight: 17.24 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
  • Canonical: Q9UUB1 (Residues: 1-159; Coverage: 100%)
Gene names: SPBC409.13, rib4
Sequence domains: 6,7-dimethyl-8-ribityllumazine synthase
Structure domains: Lumazine/riboflavin synthase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 111.758Å b: 145.504Å c: 129.127Å
α: 90° β: 90° γ: 90°
R R work R free
0.198 0.194 0.231
Expression system: Escherichia coli