1ky5

X-ray diffraction
2.8Å resolution

D244E mutant S-Adenosylhomocysteine hydrolase refined with noncrystallographic restraints

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.48 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10760 (Residues: 2-432; Coverage: 100%)
Gene name: Ahcy
Sequence domains: S-adenosyl-L-homocysteine hydrolase, NAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 4 x NAI
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 91Å b: 223Å c: 91.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.215 0.293
Expression system: Escherichia coli