› 1kxj

X-ray diffraction
2.8Å resolution

The Crystal Structure of Glutamine Amidotransferase from Thermotoga maritima

Released:
DOI: 10.2210/pdb1kxj/pdb
PDB entry 1kxj coloured by chain, front view.
PDB entry 1kxj coloured by chain, side view.
PDB entry 1kxj coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of glutamine amidotransferase from Thermotoga maritima.
Korolev S, Skarina T, Evdokimova E, Beasley S, Edwards A, Joachimiak A, Savchenko A
Proteins 49 420-2 (2002)
PMID: 12360532

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazole glycerol phosphate synthase subunit HisH Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 23.47 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0C8 (Residues: 1-201; Coverage: 100%)
Gene names: TM_1038, hisH
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 82.045Å b: 82.045Å c: 176.352Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.274
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Conformational changes in ammonia-channeling glutamine amidotransferases.
Mouilleron et al. (2007)
1 more

2 mentions without citation

Towards fully automated structure-based function prediction in structural genomics: a case study.
Watson et al. (2007)
1 more