› 1kwg

X-ray diffraction
1.6Å resolution

Crystal structure of Thermus thermophilus A4 beta-galactosidase

Released:
DOI: 10.2210/pdb1kwg/pdb
PDB entry 1kwg coloured by chain, front view.
PDB entry 1kwg coloured by chain, side view.
PDB entry 1kwg coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Trimeric crystal structure of the glycoside hydrolase family 42 beta-galactosidase from Thermus thermophilus A4 and the structure of its complex with galactose.
Hidaka M, Fushinobu S, Ohtsu N, Motoshima H, Matsuzawa H, Shoun H, Wakagi T
J Mol Biol 322 79-91 (2002)
PMID: 12215416

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 645 amino acids
Theoretical weight: 72.91 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: O69315 (Residues: 1-645; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand ACT 2 x ACT
Ligand ZN 1 x ZN
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P321
Unit cell:
a: 97.737Å b: 97.737Å c: 129.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.184
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Galacto-oligosaccharide production using microbial beta-galactosidase: current state and perspectives.
Park et al. (2010)
4 more

9 mentions without citation

Cold-Active β-Galactosidases: Insight into Cold Adaption Mechanisms and Biotechnological Exploitation.
Mangiagalli et al. (2021)
8 more