› 1kwf

X-ray diffraction
0.94Å resolution

Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate

Released:
DOI: 10.2210/pdb1kwf/pdb
PDB entry 1kwf coloured by chain, front view.
PDB entry 1kwf coloured by chain, side view.
PDB entry 1kwf coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate.
Guérin DM, Lascombe MB, Costabel M, Souchon H, Lamzin V, Béguin P, Alzari PM
J Mol Biol 316 1061-9 (2002)
PMID: 11884144

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase A Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.33 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DC29 (Residues: 33-395; Coverage: 82%)
Gene names: Cthe_0269, celA
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand BGC 3 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 49.336Å b: 62.767Å c: 103.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.094 not available 0.113
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Xylanases, xylanase families and extremophilic xylanases.
Collins et al. (2005)
11 more

14 mentions without citation

CH/π Interactions in Carbohydrate Recognition.
Spiwok V. (2017)
13 more