X-ray diffraction
2.7Å resolution

Crystal Structure of the Anti-His Tag Antibody 3D5 Single-Chain Fragment (scFv) in Complex with a Oligohistidine peptide


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-his tag antibody 3d5 variable light chain, Peptide linker, Anti-his tag antibody 3d5 variable heavy chain Chain: L
Molecule details ›
Chain: L
Length: 250 amino acids
Theoretical weight: 26.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Oligohistidine peptide Antigen Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 847 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P3221
Unit cell:
a: 106.51Å b: 106.51Å c: 92.8Å
α: 90° β: 90° γ: 120°
R R work R free
0.19 0.19 0.222
Expression systems:
  • Escherichia coli
  • Not provided