1ksp

X-ray diffraction
2.3Å resolution

DNA polymerase I Klenow fragment (E.C.2.7.7.7) mutant/DNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00582 (Residues: 325-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains: DNA polymerase family A
Structure domains:
DNA (5'-D(P*TP*TP*PST)-3') Chain: B
Molecule details ›
Chain: B
Length: 3 nucleotides
Theoretical weight: 884 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43
Unit cell:
a: 101.56Å b: 101.56Å c: 85.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.241
Expression systems:
  • Escherichia coli
  • Not provided