1kqp

X-ray diffraction
1.03Å resolution

NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS AT 1 A RESOLUTION

Released:
Source organism: Bacillus subtilis
Primary publication:
NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 A resolution.
Acta Crystallogr. D Biol. Crystallogr. 58 1138-46 (2002)
PMID: 12077433

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08164 (Residues: 2-272; Coverage: 100%)
Gene names: BSU03130, nadE, outB
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 52.28Å b: 84.79Å c: 59.64Å
α: 90° β: 110.5° γ: 90°
R-values:
R R work R free
0.108 0.106 0.135
Expression system: Escherichia coli