› 1kqj

X-ray diffraction
1.7Å resolution

Crystal Structure of a Mutant of MutY Catalytic Domain

Released:
DOI: 10.2210/pdb1kqj/pdb
PDB 1kqj coloured by chain and viewed from the front.
PDB 1kqj coloured by chain and viewed from the side.
PDB 1kqj coloured by chain and viewed from the top.
Source organism: Escherichia coli
Entry authors: Messick TE, Chmiel NH, Golinelli MP, David SS, Joshua-Tor L

Function and Biology Details

Reaction catalysed: 
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17802 (Residues: 1-225; Coverage: 64%)
Gene names: JW2928, b2961, micA, mutY
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand SF4 1 x SF4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 83.55Å b: 49.9Å c: 71.01Å
α: 90° β: 122.56° γ: 90°
R-values:
R R work R free
0.191 0.191 0.208
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Repair of 8-oxoG:A mismatches by the MUTYH glycosylase: Mechanism, metals and medicine.
Banda et al. (2017)
4 more

1 mention without citation

Bidirectional Electron-Transfer in Polypeptides with Various Secondary Structures.
Han et al. (2017)