1kq2

X-ray diffraction
2.71Å resolution

Crystal Structure of an Hfq-RNA Complex

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Hfq Chains: A, B, H, I, K, M
Molecule details ›
Chains: A, B, H, I, K, M
Length: 77 amino acids
Theoretical weight: 8.79 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JV59 (Residues: 1-77; Coverage: null%)
Gene names: SAV1305, hfq
Structure domains: SH3 type barrels.
5'-R(*AP*UP*UP*UP*UP*UP*G)-3' Chain: R
Molecule details ›
Chain: R
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2221
Unit cell:
a: 80.87Å b: 115.6Å c: 101.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.266
Expression systems:
  • Escherichia coli
  • Not provided