X-ray diffraction
2.5Å resolution

Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SUMO-conjugating enzyme UBC9 Chains: A, C
Molecule details ›
Chains: A, C
Length: 159 amino acids
Theoretical weight: 18.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P63279 (Residues: 1-158; Coverage: 100%)
Gene names: UBC9, UBCE9, UBE2I
Structure domains: Ubiquitin Conjugating Enzyme
Ran GTPase-activating protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 171 amino acids
Theoretical weight: 18.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P46061 (Residues: 420-589; Coverage: 29%)
Gene names: Fug1, Rangap1
Structure domains: Ran-GTPase activating protein 1, C-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 86.531Å b: 126.509Å c: 72.639Å
α: 90° β: 90° γ: 90°
R R work R free
0.223 0.223 0.3
Expression system: Escherichia coli