1kpk

X-ray diffraction
3.5Å resolution

Crystal Structure of the ClC Chloride Channel from E. coli

Released:
Source organism: Escherichia coli
Entry authors: Dutzler R, Campbell EB, Cadene M, Chait BT, MacKinnon R

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
H(+)/Cl(-) exchange transporter ClcA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 473 amino acids
Theoretical weight: 50.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P212121
Unit cell:
a: 105.66Å b: 152.525Å c: 263.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.29 0.301
Expression system: Escherichia coli BL21(DE3)