› 1ko7

X-ray diffraction
1.95Å resolution

X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution

Released:
DOI: 10.2210/pdb1ko7/pdb
PDB 1ko7 coloured by chain and viewed from the front.
PDB 1ko7 coloured by chain and viewed from the side.
PDB 1ko7 coloured by chain and viewed from the top.
Source organism: Staphylococcus xylosus
Primary publication:
Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions.
Márquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K
Proc. Natl. Acad. Sci. U.S.A. 99 3458-63 (2002)
PMID: 11904409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HPr kinase/phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.37 KDa
Source organism: Staphylococcus xylosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S1H5 (Residues: 1-314; Coverage: 100%)
Gene name: hprK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 152.24Å b: 152.24Å c: 194.686Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.246
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bacterial tyrosine kinases: evolution, biological function and structural insights.
Grangeasse et al. (2012)
8 more

1 mention without citation

Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions.
Márquez et al. (2002)