X-ray diffraction
1.9Å resolution

Crystal Structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase Complexed with Catechol under Anaerobic Condition


Function and Biology Details

Reaction catalysed:
Biphenyl-2,3-diol + O(2) = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Biphenyl-2,3-diol 1,2-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.38 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Burkholderia cepacia
  • Canonical: P47228 (Residues: 2-298; Coverage: 100%)
Gene names: Bxe_C1191, Bxeno_C1125, bphC
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 122.8Å b: 122.8Å c: 110.6Å
α: 90° β: 90° γ: 90°
R R work R free
0.201 0.164 0.193
Expression system: Burkholderia cepacia