› 1kna

X-ray diffraction
2.1Å resolution

Chromo domain of HP1 complexed with histone H3 tail containing dimethyllysine 9.

Released:
DOI: 10.2210/pdb1kna/pdb
PDB entry 1kna coloured by chain, front view.
PDB entry 1kna coloured by chain, side view.
PDB entry 1kna coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail.
Jacobs SA, Khorasanizadeh S
Science 295 2080-3 (2002)
PMID: 11859155

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135450 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heterochromatin protein 1 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.59 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P05205 (Residues: 17-76; Coverage: 29%)
Gene names: CG8409, HP1, HP1a, Su(var)2-5, Su(var)205
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 1.76 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P02299 (Residues: 2-17; Coverage: 12%)
Gene names: CG31613, CG33803, CG33806, CG33809, CG33812, CG33815, CG33818, CG33821, CG33824, CG33827, CG33830, CG33833, CG33836, CG33839, CG33842, CG33845, CG33848, CG33851, CG33854, CG33857, CG33860, CG33863, CG33866, His3, His3:CG31613, His3:CG33803, His3:CG33806, His3:CG33809, His3:CG33812, His3:CG33815, His3:CG33818, His3:CG33821, His3:CG33824, His3:CG33827, His3:CG33830, His3:CG33833, His3:CG33836, His3:CG33839, His3:CG33842, His3:CG33845, His3:CG33848, His3:CG33851, His3:CG33854, His3:CG33857, His3:CG33860, His3:CG33863, His3:CG33866

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 33.792Å b: 76.883Å c: 75.548Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

150 review citations

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
149 more

17 mentions without citation

Chromatin dynamics: interplay between remodeling enzymes and histone modifications.
Swygert et al. (2014)
16 more