1kmk

X-ray diffraction
2.2Å resolution

E. coli NifS/CsdB protein at 2.20A with the cysteine perselenide intermediate (residue CSZ).

Released:

Function and Biology Details

Reactions catalysed:
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 125.855Å b: 125.855Å c: 133.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.239
Expression system: Escherichia coli