› 1kj8

X-ray diffraction
1.6Å resolution

Crystal Structure of PurT-Encoded Glycinamide Ribonucleotide Transformylase in Complex with Mg-ATP and GAR

Released:
DOI: 10.2210/pdb1kj8/pdb
PDB entry 1kj8 coloured by chain, front view.
PDB entry 1kj8 coloured by chain, side view.
PDB entry 1kj8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site.
Thoden JB, Firestine SM, Benkovic SJ, Holden HM
J Biol Chem 277 23898-908 (2002)
PMID: 11953435

Function and Biology Details

Reaction catalysed: 
ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + phosphate + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate-dependent phosphoribosylglycinamide formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 42.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P33221 (Residues: 2-392; Coverage: 100%)
Gene names: JW1838, b1849, purT
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand MG 4 x MG
Ligand NA 2 x NA
Ligand CL 4 x CL
Ligand ATP 2 x ATP
Ligand GAR 2 x GAR
Ligand MPO 1 x MPO
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 62.1Å b: 179.3Å c: 75.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
3 more

4 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
3 more