X-ray diffraction
2.6Å resolution

D100E trichodiene synthase complexed with pyrophosphate


Function and Biology Details

Reaction catalysed:
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Trichodiene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 44.06 KDa
Source organism: Fusarium sporotrichioides
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P13513 (Residues: 1-374; Coverage: 100%)
Gene names: TOX 5, TRI5
Sequence domains: Trichodiene synthase (TRI5)
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 122.723Å b: 122.723Å c: 151.01Å
α: 90° β: 90° γ: 120°
R R work R free
0.219 0.219 0.252
Expression system: Escherichia coli BL21(DE3)