› 1kix

X-ray diffraction
2.7Å resolution

Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA

Released:
DOI: 10.2210/pdb1kix/pdb
PDB entry 1kix coloured by chain, front view.
PDB entry 1kix coloured by chain, side view.
PDB entry 1kix coloured by chain, top view.
Source organism: Sterkiella nova
Primary publication:
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA.
Peersen OB, Ruggles JA, Schultz SC
Nat Struct Biol 9 182-7 (2002)
PMID: 11836536

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114083 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Telomere-binding protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 56.17 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P29549 (Residues: 1-495; Coverage: 100%)
Gene names: MAC-56A, MAC-56K, MAC-56S
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
5'-D(*T*TP*TP*TP*GP*GP*GP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: Sterkiella nova
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: F432
Unit cell:
a: 284Å b: 284Å c: 284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
6 more

1 mention without citation

Nucleotide shuffling and ssDNA recognition in Oxytricha nova telomere end-binding protein complexes.
Theobald et al. (2003)