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Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria.

Gáspári Z, Patthy A, Gráf L, Perczel A
Eur J Biochem 269 527-37 (2002)
Related entries: 1kgm, 1kj0

Cited: 28 times
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Abstract

The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors.

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  2. Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. Héja D, Harmat V, Fodor K, Wilmanns M, Dobó J, Kékesi KA, Závodszky P, Gál P, Pál G. J Biol Chem 287 20290-20300 (2012)
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  15. Molecular identification of SGPP-5, a novel pacifastin-like peptide precursor in the desert locust. Simonet G, Claeys I, Van Soest S, Breugelmans B, Franssens V, De Loof A, Vanden Broeck J. Peptides 25 941-950 (2004)
  16. PtPLC, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab Portunus trituberculatus. Liu Y, Cui Z, Shi G, Luo D, Wang S, Wang C. Fish Shellfish Immunol 43 36-42 (2015)
  17. A shrimp pacifastin light chain-like inhibitor: molecular identification and role in the control of the prophenoloxidase system. Sangsuriya P, Charoensapsri W, Chomwong S, Senapin S, Tassanakajon A, Amparyup P. Dev Comp Immunol 54 32-45 (2016)
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  20. Identification of a small pacifastin protease inhibitor from Nasonia vitripennis venom that inhibits humoral immunity of host (Musca domestica). Qian C, Liang D, Liu Y, Wang P, Kausar S, Wei G, Zhu B, Wang L, Liu C. Toxicon 131 54-62 (2017)
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  22. Kinetic analysis, expression pattern, and production of a recombinant fungal protease inhibitor of tasar silkworm Antheraea mylitta. Roy S, Ravipati VR, Ghorai S, Chakrabarti M, Das AK, Ghosh AK. Appl Biochem Biotechnol 168 1076-1085 (2012)


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