› 1kig

X-ray diffraction
3Å resolution

BOVINE FACTOR XA

Released:
DOI: 10.2210/pdb1kig/pdb
PDB entry 1kig coloured by chain, front view.
PDB entry 1kig coloured by chain, side view.
PDB entry 1kig coloured by chain, top view.
Source organisms:
Primary publication:
Unexpected binding mode of tick anticoagulant peptide complexed to bovine factor Xa.
Wei A, Alexander RS, Duke J, Ross H, Rosenfeld SA, Chang CH
J Mol Biol 283 147-54 (1998)
PMID: 9761680

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133221 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Activated factor Xa heavy chain Chain: H
Molecule details ›
Chain: H
Length: 241 amino acids
Theoretical weight: 26.85 KDa
Source organism: Bos taurus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00743 (Residues: 234-474; Coverage: 51%)
Gene name: F10
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Factor X light chain Chain: L
Molecule details ›
Chain: L
Length: 51 amino acids
Theoretical weight: 5.63 KDa
Source organism: Bos taurus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00743 (Residues: 129-179; Coverage: 11%)
Gene name: F10
Sequence domains: Coagulation Factor Xa inhibitory site
Structure domains: Laminin
Tick anticoagulant peptide Chain: I
Molecule details ›
Chain: I
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Ornithodoros moubata
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P17726 (Residues: 1-60; Coverage: 100%)
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42212
Unit cell:
a: 133.1Å b: 133.1Å c: 68.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 not available
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 review citations

The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms.
Fry et al. (2009)
8 more

5 mentions without citation

Demonstration of the extrinsic coagulation pathway in teleostei: identification of zebrafish coagulation factor VII.
Sheehan et al. (2001)
4 more