1kf9

X-ray diffraction
2.6Å resolution

PHAGE DISPLAY DERIVED VARIANT OF HUMAN GROWTH HORMONE COMPLEXED WITH TWO COPIES OF THE EXTRACELLULAR DOMAIN OF ITS RECEPTOR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Somatotropin Chains: A, D
Molecule details ›
Chains: A, D
Length: 191 amino acids
Theoretical weight: 21.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01241 (Residues: 27-217; Coverage: 100%)
Gene name: GH1
Structure domains: Growth Hormone; Chain: A;
Growth hormone-binding protein Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 238 amino acids
Theoretical weight: 27.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10912 (Residues: 19-256; Coverage: 38%)
Gene name: GHR
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 67.289Å b: 111.937Å c: 95.29Å
α: 90° β: 90.06° γ: 90°
R-values:
R R work R free
0.234 0.234 0.326
Expression system: Escherichia coli