Function and Biology Details
Reaction catalysed:
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
- not assigned
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-deoxyerythronolide-B synthase EryA3, modules 5 and 6
Molecule details ›
Chains: A, B, C
Length: 300 amino acids
Theoretical weight: 32.11 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase
Length: 300 amino acids
Theoretical weight: 32.11 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q03133 (Residues: 2893-3172; Coverage: 9%)
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 5.0.2
Spacegroup:
P3121
Expression system: Escherichia coli BL21
