› 1ken

X-ray diffraction
3.5Å resolution

INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH AN ANTIBODY THAT PREVENTS THE HEMAGGLUTININ LOW PH FUSOGENIC TRANSITION

Released:
DOI: 10.2210/pdb1ken/pdb
PDB 1ken coloured by chain and viewed from the front.
PDB 1ken coloured by chain and viewed from the side.
PDB 1ken coloured by chain and viewed from the top.
Source organisms:
Primary publication:
An antibody that prevents the hemagglutinin low pH fusogenic transition.
Barbey-Martin C, Gigant B, Bizebard T, Calder LJ, Wharton SA, Skehel JJ, Knossow M
Virology 294 70-4 (2002)
PMID: 11886266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
Non-polymer only decamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 328 amino acids
Theoretical weight: 36.07 KDa
Source organism: Influenza A virus (A/X-31(H3N2))
UniProt:
  • Canonical: P03438 (Residues: 17-344; Coverage: 60%)
Gene name: HA
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 175 amino acids
Theoretical weight: 20.21 KDa
Source organism: Influenza A virus (A/X-31(H3N2))
UniProt:
  • Canonical: P03437 (Residues: 346-520; Coverage: 32%)
Gene name: HA
Structure domains: Hemagglutinin Ectodomain; Chain B
Immunoglobulin kappa constant Chains: L, U
Molecule details ›
Chains: L, U
Length: 213 amino acids
Theoretical weight: 23.28 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-105; Coverage: 98%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-1 chain C region, membrane-bound form Chains: H, T
Molecule details ›
Chains: H, T
Length: 221 amino acids
Theoretical weight: 24.35 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01869 (Residues: 1-101; Coverage: 26%)
Gene names: Igh-4, Ighg1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Ligand NAG 6 x NAG
Ligand MAN 3 x MAN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 143.041Å b: 315.588Å c: 97.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.323

Quick links

Citations

17 review citations

Structural Insights for Anti-Influenza Vaccine Design.
Han et al. (2019)
16 more

32 mentions without citation

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus.
Wu et al. (2017)
31 more