1kea

X-ray diffraction
2Å resolution

STRUCTURE OF A THERMOSTABLE THYMINE-DNA GLYCOSYLASE

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymine/uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 25.44 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29588 (Residues: 1-221; Coverage: 100%)
Gene name: mig
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 68.215Å b: 68.215Å c: 98.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.248
Expression system: Escherichia coli