1kcl

X-ray diffraction
1.94Å resolution

Bacillus ciruclans strain 251 Cyclodextrin glycosyl transferase mutant G179L

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.63 KDa
Source organism: Bacillus circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer : NEW Components: GLC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 114.714Å b: 109.473Å c: 65.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.171 0.19
Expression system: Escherichia coli