1kcf

X-ray diffraction
2.3Å resolution

Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cruciform cutting endonuclease 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 30.25 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10423 (Residues: 1-258; Coverage: 100%)
Gene names: SPAC25G10.02, cce1, ydc2
Sequence domains: Mitochondrial resolvase Ydc2 / RNA splicing MRS1
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 72Å b: 133.94Å c: 73.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.238 0.27
Expression system: Escherichia coli BL21(DE3)