1kc3

X-ray diffraction
2.7Å resolution

Crystal structure of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD) in complex with NADPH and dTDP-L-rhamnose

Released:

Function and Biology Details

Reaction catalysed:
dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-4-dehydrorhamnose reductase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.59 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P26392 (Residues: 1-299; Coverage: 100%)
Gene names: STM2096, rfbD, rmlD
Sequence domains: RmlD substrate binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 47.98Å b: 72.261Å c: 82.682Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.191 0.289
Expression system: Escherichia coli