› 1k7w

X-ray diffraction
1.96Å resolution

Crystal Structure of S283A Duck Delta 2 Crystallin Mutant

Released:
DOI: 10.2210/pdb1k7w/pdb
PDB entry 1k7w coloured by chain, front view.
PDB entry 1k7w coloured by chain, side view.
PDB entry 1k7w coloured by chain, top view.
Source organism: Anas platyrhynchos
Primary publication:
Mutational analysis of duck delta 2 crystallin and the structure of an inactive mutant with bound substrate provide insight into the enzymatic mechanism of argininosuccinate lyase.
Sampaleanu LM, Yu B, Howell PL
J Biol Chem 277 4166-75 (2002)
PMID: 11698398

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 468 amino acids
Theoretical weight: 51.74 KDa
Source organism: Anas platyrhynchos
Expression system: Escherichia coli
UniProt:
  • Canonical: P24058 (Residues: 1-468; Coverage: 100%)
Gene name: ASL2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AS1 4 x AS1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 93.759Å b: 98.637Å c: 106.147Å
α: 90° β: 101.3° γ: 90°
R-values:
R R work R free
0.224 0.207 0.253
Expression system: Escherichia coli

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Citations

2 review citations

Argininosuccinic aciduria: Recent pathophysiological insights and therapeutic prospects.
Baruteau et al. (2019)
1 more