› 1k78

X-ray diffraction
2.25Å resolution

Pax5(1-149)+Ets-1(331-440)+DNA

Released:
DOI: 10.2210/pdb1k78/pdb
PDB 1k78 coloured by chain and viewed from the front.
PDB 1k78 coloured by chain and viewed from the side.
PDB 1k78 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural studies of Ets-1/Pax5 complex formation on DNA.
Garvie CW, Hagman J, Wolberger C
Mol Cell 8 1267-76 (2001)
PMID: 11779502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero pentamer
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Paired box protein Pax-5 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 149 amino acids
Theoretical weight: 16.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02548 (Residues: 1-149; Coverage: 38%)
Gene name: PAX5
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Protein C-ets-1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 110 amino acids
Theoretical weight: 12.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27577 (Residues: 331-440; Coverage: 25%)
Gene names: Ets-1, Ets1
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Pax5/Ets Binding Site on the mb-1 promoter Chains: C, G
Molecule details ›
Chains: C, G
Length: 27 nucleotides
Theoretical weight: 8.37 KDa
Pax5/Ets Binding Site on the mb-1 promoter Chains: D, H
Molecule details ›
Chains: D, H
Length: 27 nucleotides
Theoretical weight: 8.23 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D, NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 77.84Å b: 89.75Å c: 170.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.227 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Are Pax proteins potential therapeutic targets in kidney disease and cancer?
Grimley et al. (2018)
23 more

13 mentions without citation

Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition.
Cooper et al. (2014)
12 more