1k77

X-ray diffraction
1.63Å resolution

Crystal Structure of EC1530, a Putative Oxygenase from Escherichia coli

Released:

Function and Biology Details

Reaction catalysed:
2-dehydro-D-erythronate = 3-dehydro-D-erythronate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxo-tetronate isomerase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46891 (Residues: 1-258; Coverage: 100%)
Gene names: JW2709, b2739, otnI, ygbM
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 104.907Å b: 74.368Å c: 39.376Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.196 0.194 0.214
Expression system: Escherichia coli