1k6o

X-ray diffraction
3.19Å resolution

Crystal Structure of a Ternary SAP-1/SRF/c-fos SRE DNA Complex

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of a ternary SAP-1/SRF/c-fos SRE DNA complex.
J. Mol. Biol. 314 495-506 (2001)
PMID: 11846562

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
ETS domain-containing protein Elk-4 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28324 (Residues: 1-93; Coverage: 22%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Serum response factor Chains: B, C
Molecule details ›
Chains: B, C
Length: 103 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11831 (Residues: 133-235; Coverage: 20%)
Gene name: SRF
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
5'-D(*CP*AP*CP*AP*GP*GP*AP*TP*GP*TP*CP*CP*AP*TP*AP*TP*TP*AP*GP*GP*AP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.07 KDa
5'-D(*TP*GP*TP*CP*CP*TP*AP*AP*TP*AP*TP*GP*GP*AP*CP*AP*TP*CP*CP*TP*GP*TP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 23 nucleotides
Theoretical weight: 7.05 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P63
Unit cell:
a: 161.784Å b: 161.784Å c: 41.224Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.276
Expression system: Escherichia coli