1k5s

X-ray diffraction
2.43Å resolution

PENICILLIN ACYLASE, MUTANT COMPLEXED WITH PPA

Released:

Function and Biology Details

Reaction catalysed:
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Penicillin G acylase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 26-234; Coverage: 25%)
Gene name: pac
Structure domains:
Penicillin G acylase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 557 amino acids
Theoretical weight: 62.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06875 (Residues: 290-846; Coverage: 68%)
Gene name: pac
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P1
Unit cell:
a: 50.82Å b: 64.36Å c: 64.75Å
α: 72.89° β: 74.01° γ: 73.55°
R-values:
R R work R free
0.194 0.173 0.233
Expression system: Escherichia coli