1k4d

X-ray diffraction
2.3Å resolution

Potassium Channel KcsA-Fab complex in low concentration of K+

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig gamma-1 chain C region secreted form Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01868 (Residues: 1-101; Coverage: 31%)
Gene names: Igh-4, Ighg1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-105; Coverage: 98%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.21 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4
Unit cell:
a: 155.29Å b: 155.29Å c: 75.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.235
Expression system: Escherichia coli