› 1k3f

X-ray diffraction
2.5Å resolution

Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice

Released:
DOI: 10.2210/pdb1k3f/pdb
PDB entry 1k3f coloured by chain, front view.
PDB entry 1k3f coloured by chain, side view.
PDB entry 1k3f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice.
Mikhailov AM, Popov AN, Blagova EV, Smirnova EA, Vainshtein BK, Mao C, Armstrong ShR, Ealick SE, Komissarov AA
FEBS Lett 367 183-7 (1995)
PMID: 7796917

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 92.6Å b: 98.8Å c: 93.7Å
α: 90° β: 120.2° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Purine nucleoside phosphorylases: properties, functions, and clinical aspects.
Bzowska et al. (2000)
2 more

4 mentions without citation

Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.
Xia et al. (2007)
3 more