1k02

X-ray diffraction
2.7Å resolution

Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
The role of glutamine 114 in old yellow enzyme.
J. Biol. Chem. 277 2138-45 (2002)
PMID: 11668181

Function and Biology Details

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 44.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02899 (Residues: 2-400; Coverage: 100%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 142.455Å b: 142.455Å c: 42.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.143 not available
Expression system: Escherichia coli