1jzy

X-ray diffraction
3.5Å resolution

Structural Basis for the Interaction of Antibiotics with the Peptidyl Transferase Center in Eubacteria

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
50S ribosomal protein L4 Chain: K
Molecule details ›
Chain: K
Length: 205 amino acids
Theoretical weight: 22.31 KDa
Source organism: Deinococcus radiodurans
UniProt:
  • Canonical: Q9RXK1 (Residues: 1-205; Coverage: 100%)
Gene names: DR_0312, rplD
Sequence domains: Ribosomal protein L4/L1 family
50S ribosomal protein L22 Chain: L
Molecule details ›
Chain: L
Length: 134 amino acids
Theoretical weight: 15.19 KDa
Source organism: Deinococcus radiodurans
UniProt:
  • Canonical: Q9RXJ7 (Residues: 1-134; Coverage: 100%)
Gene names: DR_0316, rplV
Sequence domains: Ribosomal protein L22p/L17e
50S ribosomal protein L32 Chain: M
Molecule details ›
Chain: M
Length: 60 amino acids
Theoretical weight: 6.81 KDa
Source organism: Deinococcus radiodurans
UniProt:
  • Canonical: P49228 (Residues: 1-60; Coverage: 100%)
Gene names: DR_2366, rpmF
Sequence domains: Ribosomal L32p protein family
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 2880 nucleotides
Theoretical weight: 933.41 KDa
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I222
Unit cell:
a: 169.2Å b: 410Å c: 695Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.268 0.301