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X-ray diffraction
1.5Å resolution

Pseudomonas aeruginosa Oxidized Azurin(Cu2+) Ru(tpy)(phen)(His83)

Released:
Source organism: Pseudomonas aeruginosa
Primary publication:
Electron tunneling in single crystals of Pseudomonas aeruginosa azurins.
J. Am. Chem. Soc. 123 11623-31 (2001)
PMID: 11716717

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 52.776Å b: 61.441Å c: 75.956Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.22
Expression system: Escherichia coli BL21(DE3)