› 1jye

X-ray diffraction
1.7Å resolution

Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution

Released:
DOI: 10.2210/pdb1jye/pdb
PDB 1jye coloured by chain and viewed from the front.
PDB 1jye coloured by chain and viewed from the side.
PDB 1jye coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structure of a variant of lac repressor with increased thermostability and decreased affinity for operator.
Bell CE, Barry J, Matthews KS, Lewis M
J. Mol. Biol. 313 99-109 (2001)
PMID: 11601849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 37.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-349; Coverage: 97%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4122
Unit cell:
a: 87.007Å b: 87.007Å c: 224.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biomolecular Assemblies: Moving from Observation to Predictive Design.
Wilson et al. (2018)
2 more

2 mentions without citation

Engineering an allosteric transcription factor to respond to new ligands.
Taylor et al. (2016)
1 more